He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. Bartok O., Teesalu M., Pandey V., Hanan M., Poukkula M., Havula E., Moussaieff A., Vodala S., Nahmias Y., Kadener S.* and Hietakangas V.* Cabut-dependent repressive branch of the sugar sensing transcriptional network regulates glyceroneogenesis. In the Bandopadhay Lab, Marissa is working to study drivers of Diffuse Midline Gliomas, including the mechanisms through which MYC isoforms are activated. Are circRNAs involved in neurodevelopmental and neurodegenerative diseases? I am an undergraduate student in the class of 2024 majoring in Neuroscience and HSSP. * Corresponding authors. Pamudurti N., Bartok O., Jens O., Ashwal-Fluss R., Stottmeister C., Ruhe L., Hanan M., Wyler E., Perez-Hernandez D., Ramberger E., Shenzis S., Samson M., Dittmar G., Landthaler M., Chekulaeva M., Rajewsky N. and Kadener S. Translation of circRNAs. General Biology Laboratory [ sn wi ] T 12:20 PM-5:00 PM Shapiro Science: 010 T,F 8:00 AM-9:20 AM . Pvf1 produced by yki-gut tumors activates MEK/ERK signaling and enhances catabolism in multiple tissues. Perelman School of Medicine at the University of Pennsylvania Two of these RNAs act as miRNA sponges, but no function is known for the thousands of other circRNAs found in species across the animal kingdom. Anna developed a fascination for cancer biology while working towards her bachelors degree at Lund University, Sweden. Now the stage is set for the most exciting questions, which we are currently working in the lab, by combining molecular, computational and RNA biology with advances techniques in genetics and neurosciences. We are now using this tool to establish sensors of each signaling pathway involved in gut homeostasis, and will use these reagents to examine pathway crosstalk. We have taken a number of approaches to address the spatial and temporal regulation of signaling pathway activities in the gut epithelium. Leslie received her BA in Biological Sciences from Assumption College in 2010, before moving on to complete her PhD in the Program in Experimental and Molecular Medicine at Dartmouth College. Community support Reprint. A slow RNA polymerase II affects alternative splicing in vivo. During her time at Brandeis, Maddie worked in the lab of Sebastian Kadener studying RNA metabolism and the circadian rhythm. Patop IL, Wst S, Kadener S. Craving for Introns. *, Bartok O. *, Afik S., Menet J., Wolfson V., Weissbein U., Haimovich D., Gafni C., Friedman N., Rosbash M. and Kadener S. . He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. Coordination between transcription and pre-mRNA processing. Circadian clocks are also exceptionallyplasticas they can quickly and specifically adjust to specific environmental cues. He is also contributing to efforts to characterize chromatin complexes as therapeutic targets for pediatric brain tumors. I am studying potential applications of the CRISPR/Cas13 system. Now, in an article published in the journal Molecular Cell, the lab of Dr. Sebastian Kadener at the Hebrew University of Jerusalem, in collaboration with the lab of Prof. Nikolaus Rajewsky at the . Portal D., Lobo G.S., Kadener S., Prasad J., Espinosa J.M., Pereira C.A., Tang Z., Lin R.J., Manley J.L., Kornblihtt A.R., Flawia M.M. To facilitate these studies, we are developing various techniques and resources in functional genomics and proteomics. Molecular Neurobiology and RNA metabolism. Pathways that are misregulated in animals that develop organ wasting in the context of these various tumor models are identified using a combination of transcriptomics, e.g. These tools, which are available via. 2012- Lab manager at Ami Citri laboratory at the Hebrew University of Jerusalem 2010- 2012 Postdoc at the Hebrew University of Jerusalem at Sebastian Kadener Lab; research topic-studying neodegenerative diseases using fruit flies as a model 2000-2003 Research assistant at QBI company; I was part of research Hanan M, Simchovitz A, Yayon N, Vaknine S, Cohen-Fultheim R, Karmon M, Madrer N, Rohrlich TM, Maman M, Bennett ER, Greenberg DS, Meshorer E, Levanon EY, Soreq H, Kadener S. Past, present, and future of circRNAs. Hanan M., Soreq H. and Kadener S. CircRNAs in the brain. A slow RNA polymerase II affects alternative splicing in vivo. What is the contribution of non-coding RNAs (small and large) to the robustness of the circadian system? Statuses. Circadian clocks are also exceptionallyplasticas they can quickly and specifically adjust to specific environmental cues. While at Caltech, Adam studied luminescent ruthenium complexes that function as light switch probes for DNA mismatches. Our laboratory is interested for understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. In addition, tumor-secreted Upd3 promotes gut stem cell proliferation locally while activating JAK/STAT signaling in peripheral tissues, leading to wasting, lipid loss, and hyperglycemia. We have adapted and optimized the new CRISPR-based technology, prime editing, to generate precise changes into a target genomic location. We demonstrated that shRNAs are more efficient and specific than long dsRNA when expressed as transgenes, and generated a genome scale collection of >13,000 lines covering 75% of the genome in our optimized VALIUM vectors (85% of highly conserved genes). Article. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. Nogues G., Kadener S., Cramer P., Bentley D. and Kornblihtt A.R. We have several efforts underway to produce new binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and QF. This dual reporter can be used in either fixed or live tissues to monitor spatio-temporal activity of signaling pathways at an unprecedented resolution. Derr A, Yang C, Zilionis R, Sergushichev A, Blodgett DM, Redick S, Bortell R, Luban J, Harlan DM, Kadener S, Greiner DL, Klein A, Artyomov MN, Garber M. End Sequence Analysis Toolkit (ESAT) expands the extractable information from single-cell RNA-seq data. We are particularly interested on the role of circular RNAs (circRNAs) at the molecular and neural levels as well as the mechanisms underlying circadian clocks. Adam grew up in Ethiopia and moved to Bremen, Germany where he received his B.Sc and M.Sci in Biochemistry and Cell Biology from Jacobs University which was his very first exposure to the exciting field of epigenetics. Aaron is an Attending Pediatric Neuro-Oncologist at the Dana-Farber/Boston Childrens Cancer and Blood Disorders Center. Nucleic Acids Research47(11): e95; 1-16 (2017). Andranik Ivanov and Sebastian Meczmack (Prof. Rajewsky's . We are now adapting the pooled-format CRISPR screening approach for use in cell lines from medically relevant mosquito species. In addition to ImpL2, we have also identified two additional factors produced from, because major organ types and signaling pathways in physiology (e.g. Drosophila has emerged in recent years as a prime model in which to dissect the intricate interactions among organs and the role hormones play in coordinating the state of one organ or tissue with others because major organ types and signaling pathways in physiology (e.g. Our laboratory is interested in understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. and Mimi is determined to introduce her to cricket and Aussie footy (AFL). Eric received his MS in biomedicine from Uppsala University, Sweden in 2010. Transcriptional activators differ in their abilities to control alternative splicing. Despite the importance of antibodies in biology study, antibodies are not available for most fly proteins. These experiments will provide a system-wide understanding of the factors originating from tumors and how they perturb systemic energy balance, and how peripheral organs in turn respond to initiate cachectic phenotypes. Further, our work and that of others has suggested functions of these moleculesin vivo. From a number of screens (receptome-wide RNAi screen, miRNA screens, etc. in English and Anthropology from Washington University in St. Louis (22). An early ancestor in the evolution of splicing: a. Nogues G., Kadener S., Cramer P., de la Mata M., Fededa J.P., Blaustein M., Srebrow A. and Kornblihtt A.R. * Corresponding authors. Or do you know how to improve StudyLib UI? Prior to coming to Brandeis, Sayan worked in the Bangalore Life Science Cluster, India as a research fellow. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. He went to college in Malaysia before moving to Philadelphia. 1 / 3 Looking for Postdoctoral researchers We are also helping the, Characterizing the proteome composition of organelles and subcellular regions of living cells can facilitate the understanding of cellular organization as well as protein interactome networks. Strikingly, these phenotypes are reminiscent of the pathophysiology of cystic fibrosis (CF), as in CF the non-functional CF transmembrane conductance regulator (CFTR) increases ENaC activity resulting in chronic dehydration of the intraluminal surface liquid in organs such as kidney, colon, lung and sweat glands. Rosi is attending the structural MS workshop in Finland. Dana joined the Bandopadhayay Lab in 2021 where is she working to apply single-cell methods to evaluate the heterogeneity of pediatric gliomas, and to understand spatial associations. Additional unpublished work from my lab demonstrates that a subset of circRNAs is translated. Community support Reagent. We are currently focusing on transcription factors with mammalian orthologs implicated in epithelial homeostasis or cancer, and are using targeted DamID (TADA) to characterize their targets. Copyright 2017 | Privacy policy | Legal statements
Regulation of alternative splicing by a transcriptional enhancer through RNA pol II elongation. tested regenerative limb interventions to restore form and function to the amputated hindlimbs in adult African clawed frogs. Molecular Neurobiology and RNA Metabolism Laboratory. I am analyzing the effect of circRNA on aging in Drosophila, majoring in biochemistry and neuroscience. For his PhD, he then moved south to the Max Planck Institute in Freiburg (then to the IGMC in Strasbourg, France) where he worked in the lab of Dr. Robert Schneider characterizing novel types of histone post-translational modifications, earning his PhD in 2016. He then pursued a PhD in chemistry at Caltech (17) under the mentorship of Dr. Jackie Barton. Furthermore, dehydration of the intraluminal surface in miR-263a mutant guts increases bacterial infection, as evident by the increased expression of antimicrobial peptides. He can also be found doing crossword puzzles at odd hours. Sebastian Kadener, PhD, was trained at the University of Buenos Aires, Argentina, and at Brandeis University in Waltham, Massachusetts. S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . She also loves sleeping in, making guacamole, and reading about art history. B.S., University of Barcelona, Spain Ph.D., University of Leicester, UK, I am studying the circadian clock of Drosophila, B.S. . Fun fact: Anna is a gardening nerd on hold, as she does not have a garden in Boston. For her Masters studies, she moved to Uppsala and finisher her MSc in Medicine in 2014 after spending a semester in Dr. Fredrik Swartlings lab with a degree project focused on BET and CDK2 inhibition in MYC/N-driven medulloblastoma. We are using CRISPR to mutate or engineer cell lines that can be used for screening, and have developed efficient protocols in Drosophila cells, which are particularly challenging as they are polyploid and difficult to grow following single-cell isolation. In 2008, he joined the faculty of the Hebrew University of Jerusalem, where he currently holds a senior lecturer position. The EMBO journal 20 (20), 5759-5768, 2001. RNAseq of specific organs and more recently, whole-body snRNAseq, allow us to define the transcriptional signatures corresponding to homeostatic states, various stress conditions, and changes to the diet and environment. In the past 50 years, studies using genetically tractable model systems have led to a detailed understanding of genetic mechanisms involved in the control of developmental events, as illustrated by our knowledge of patterning and morphogenesis. Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. We use Drosophila as a model system to characterize the mechanisms underlying communication between cells, tissues, and organs. Despite the importance of antibodies in biology study, antibodies are not available for most fly proteins. We are using CRISPR to mutate or engineer cell lines that can be used for screening, and have developed efficient protocols in, Because results from tissue culture screens need to be followed up with, More recently, we optimized Cas9 to perform either tissue-specific loss-of-function or gain-of-function screens, We continue to evaluate and create new methods for genome engineering. In the Bandopadhayay Lab, Anna will be expanding her skills to apply genomic approaches to understand the mechanisms through which gliomas develop, and to develop strategies to overcome therapeutic resistance. Proximity labeling utilizes enzymes that generate reactive radicals to covalently tag neighboring proteins. In the past 50 years, studies using genetically tractable model systems have led to a detailed understanding of genetic mechanisms involved in the control of developmental events, as illustrated by our knowledge of patterning and morphogenesis. Lic. Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. Second, we showed that the calcium channel TrpA1 is expressed in ISCs and is required for their proliferation. Alexander M. van der Linden, Matthew Beverly, Sebastian Kadener, Joseph Rodriguez, Sara Wasserman, Michael Rosbash, Piali Sengupta. Eric joined the Bandopadhayay Lab in 2020 where he is working to characterize driver mutations found in pediatric brain tumors and developing new approaches to treat these children with targeted therapies. Verified email at brandeis.edu - Homepage. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. Pandey V., Turm H., Bekenstein U., Shifman S. and Kadener S. A new. and Kornblihtt A.R. Patop IL, Wst S, Kadener S. Craving for Introns. With this advance, we hope to enable the mosquito community to easily perform genome-wide loss- or gain-of-function screens in cells, for example to discover entry and infection mechanisms of pathogens and drugs, as well as to aid in the functional annotation of mosquito genomes. Im an undergraduate in the Class of 2023 majoring in Chemical Biology at Brandeis. He also enjoys playing handball and online chess. and Torres H.N. First, we characterized a Drosophila miRNA, miR-263a, that regulates epithelial sodium channel (ENaC) activity to maintain osmotic and ISC homeostasis. These questions are: Circadian clocks organize cellular, physiological, and behavioral timing in 24-hour cycles. I am a PhD student in the lab since 2018 after graduating from The College of Wooster. Acquiring answers to these fascinating questions requires a deep understanding of the mechanisms by which cells integrate signals received from their inner selves, neighboring cells, other organs and the environment. I am researching the role of circular RNAs (circRNAs) in aging processes in Drosophila melanogaster. Studying the neuronal functions of circular RNA. Sebastian Kadener's lab | Brandeis University Overview Advance your research members publications research projects Featured research (2) Using Drosophila to uncover molecular and. Transcription factor Dam fusions of the major proliferative signaling pathways will be used to address questions about crosstalk and target co-regulation. Hanan M., Soreq H. and Kadener S. CircRNAs in the brain. Jazmin is currently investigating how circRNA levels change at synapses and whether they are modulated by neuronal activity. We chose to use peripheral blood monocytes as source material for gene expression profiling, because (a) blood is an easily accessible source of human cells and (b) monocytes constitute a homogenous blood cell population and have been shown to possess a high-amplitude circadian clock ( 24, 25) in contrast to other PBMCs, such as B or T cells. The current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops. Further, our work and that of others has suggested functions of these moleculesin vivo. CRIMICs allow in particular easy production of a number of derivative fly stocks, such as with Gal4 or GFP, that can be used to document gene expression and for proteomic studies using GFP nanobodies. Rabin A, Zaffagni M, Ashwal-Fluss R, Patop IL, Jajoo A, Shenzis S, Carmel L, Kadener S. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior. Its very important for us! Sebastian Kadener Principal Investigator M.Sc., The University of Buenos Aires, Buenos Aires, Argentina Ph.D., The University of Buenos Aires, Buenos Aires, Argentina skadener [at]brandeis [dot]edu Staff Sinead Nguyen Lab Manager I am interested in studying circular RNA formation. Chikne V.*, Doniger T.*, Bartok O. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. Our lab is interested in determining how molecular and neural circadian components regulate each other and generate a system that is both robust and plastic. Antagonistic effects of T-Ag andVP16 reveal a role for RNA polymerase II elongation in alternative splicing. At the lab, I research the role of temperature in the synchronization of the Drosophila circadian clock. We have also generated stable nuclease-dead Cas9 activator (dCas9a) cell lines that can be used, after transfection with gRNAs, to perform overexpression screens - thus complementing loss of function screens. Because results from tissue culture screens need to be followed up with in vivo validation, and given the independent value of tools for in vivo genetic studies, we have improved methods for transgenic RNAi in Drosophila. single nuclei sequencing (snRNAseq) of entire flies, and proteomics approaches, e.g. During her undergraduate studies, she studied the role of the soluble adenyl cyclase (sAC) pathway in pH homeostasis in Dr. Katie Barotts lab. Clonally stable VK allelic choice instructs IgK repertoire. and Torres H.N. Sher is now contributing to efforts to characterize FGFR alterations in pediatric gliomas, and to develop methods to optimize TP53 reactivation in Diffuse Midline Gliomas. The tagged endogenous proteins can then be isolated for further analysis by MS. To analyze protein-protein interactions or identify components that localize to discrete subcellular compartments, we developed tools based on APEX, an engineered ascorbate peroxidase derived from plants, and BioID, a mutant form of the biotin ligase BirA from E coli and demonstrated their use for in vivo studies. Bartok O., Kyriacou C., Levine J., Sehgal A. and Kadener S. Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs. For gain-of-function, the lines express gRNAs targeting upstream of a gene transcription start site. Using the DRSC screening platform, the functions of the ~15,000 predicted Drosophila genes can be systematically analyzed to address questions in cell signaling, cell morphology, host-pathogen interactions, ion channel function, and many other topics. , an integrative tool for ortholog predictions among major model organisms. and Kadener S. Mathematical Model of the, Kadener S., Menet J., Sugino K., Horwich M.D., Weissbein U., Nawathean P., Vagin V., Zamore P., Nelson S. and Rosbash M. A role for miRNAs in the, Kadener S., Rodriguez J., Abruzzi K. and Rosbash M. Genome-wide identification of targets of the, Kadener S., Schoer R., Menet J. and Rosbash M. Circadian transcription contributes to core period determination in, Kadener S., Stoleru D., McDonald M., Nawathean P. and Rosbash M. , Rosbash M., Bradley S., Kadener S., Li Y., Luo W., Menet J.S., Nagoshi E., Palm K., Schoer R., Shang Y. and Tang C.H. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. Sayan is a graduate student in the Molecular and Cell Biology program at Brandeis and he is investigating the role of circRNA in aging and neurodegeneration. DIOPT has been well received by the scientific community and has been integrated into FlyBase, PomBase, MARRVEL (Model organism Aggregated Resources for Rare Variant ExpLoration) and AGR (Alliance of Genome Resources); integrates annotation information for orthologs of multiple species in unified interfaces; allows scientists to mine the validation and phenotype data for. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. * Corresponding authors. We showed that miRNAs have a key role in providing robustness to the circadian system both during development and in adults. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. In a collaboration with Sebastian Kadeners lab at Brandeis, we generated a new set of PspCas13b and RfxCas13d expression constructs that can be used to target RNA in cells and in vivo. Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. I am interested in studying circular RNA formation. Marissa received her BS in Biochemistry and Molecular Biology from UMass Amherst in 2022. April received her BS in Cellular and Molecular Biology from Beloit College in 2017, where she worked in the lab of Dr. Amy Briggs studying the role offpgsgenes inArabidopsis thaliana. 0. Wittenbrink N, Ananthasubramaniam B, Mnch M, Koller B, Maier B, Weschke C, Bes F, de Zeeuw J, Nowozin C, Wahnschaffe A, Wisniewski S, Zaleska M, Bartok O, Ashwal-Fluss R, Lammert H, Herzel H, Hummel M, Kadener S, Kunz D, Kramer A. Levin-Klein R., Fraenkel S., Lichtenstein M., Matheson L., Bartok O., Nevo Y., Kadener S., Corcoran A., Cedar H., Bergman Y. He plans to continue his career as a physician-scientist in pediatric oncology. Sebastian Kadener. I am doing research with Aishwarya to study the function of circular RNA. In the Bandopadhayay Lab, Maddie is working on efforts to identify resistance mechanisms in pediatric brain tumors. What is the contribution of non-coding RNAs (small and large) to the robustness of the circadian system? Jess earned her PhD in Neuroscience working in the laboratory of Dr. Thomas Clandinin, utilizing the Drosophila visual system and forward genetics to study synapse development and axon degeneration. NanoTags: One of the key reagents to understand protein expression and function are antibodies. Hu, Comjean, Attrill, Antonazzo, Jim Thurmond, Li, Chao, Mohr, Brown, Perrimon From 2002 to 2008, Sebastian Kadener was a postdoc working in the Michael Rosbash laboratory. Adam then moved to Boston for his postdoc in the lab of Dr. Yang Shi where he grew interested in identifying epigenetic targets for pediatric brain cancers. Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. Lacadie S.A., Tardiff D.F., Kadener S. and Rosbash M. In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants. Chem. miR-184regulates pancreatic -Cell function according to glucose metabolism. Portal D., Lobo G.S., Kadener S., Prasad J., Espinosa J.M., Pereira C.A., Tang Z., Lin R.J., Manley J.L., Kornblihtt A.R., Flawia M.M. Fun fact: Leslie played basketball through college and is now an avid cyclist. Interestingly, JAK/STAT signaling in the muscle or fat body directly triggers the expression of ImpL2, which in turn impairs local insulin signaling and its associated energy balance. In addition to ImpL2, we have also identified two additional factors produced from yki-gut tumors that contribute to tissue wasting: the Pvr receptor tyrosine kinase ligand Pvf1 and the IL6-like cytokine Unpaired 3 (Upd3). In addition, skeletal muscles produce various myokines that influence metabolic homeostasis, lifespan, and the progression of age-related diseases and aging in non-muscle tissues. Eric studied how the interaction of cell surface proteins expressed by different cell types can regulate blood vessel formation in normal cells and tumors. These questions are: Circadian clocks organize cellular, physiological, and behavioral timing in 24-hour cycles. Patop, Ines Lucia; Kadener, Sebastian . In addition, we are extending our studies to additional tumor models. Fun fact: In his native language of Urdu, Shers full name translates to lion/tiger brave. Michael earned a BA in cellular biology and neuroscience from Rutgers University. What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? Rabin A, Zaffagni M, Ashwal-Fluss R, Patop IL, Jajoo A, Shenzis S, Carmel L, Kadener S. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior. Rosenstiel332781-736-4915skadener@brandeis.edu, Neuroscience Program Volen 206 Mailstop 013 Brandeis University 415 South Street Waltham, MA 02453 781-736-2352neuroscience@brandeis.edu. Our lab is interested in determining how molecular and neural circadian components regulate each other and generate a system that is both robust and plastic. Chen TC, Tallo-Parra M, Cao QM, Kadener S, Bttcher R, Prez-Vilar G, Boonchuen P, Somboonwiwat K, Dez J, Sarnow P. A lncRNA survey finds increases in neuroprotective LINC-PINT in Parkinson's disease substantia nigra. What are the neural and molecular basis of temperature adaptation and compensation? Simchovitz A, Hanan M, Yayon N, Lee S, Bennett ER, Greenberg DS, Kadener S, Soreq H. A Parkinson's disease CircRNAs Resource reveals a link between circSLC8A1 and oxidative stress. Metazoan MicroRNAs (2018) David P. Bartel CELL Non-canonical functions of microRNA pathway enzymes - Drosha, DGCR8, Dicer and Ago proteins Selected for Faculty of 1000 (Factor 3.0 Recommended). Cramer P., Srebrow A., Kadener S., Werbajh S., de la Mata M., Melen G., Nogues G. and Kornblihtt AR. Our Funds. We continue to evaluate and create new methods for genome engineering. As an underlying mechanism, the balance or critical reaction hypothesis have been proposed. DIOPT has been well received by the scientific community and has been integrated into FlyBase, PomBase, MARRVEL (Model organism Aggregated Resources for Rare Variant ExpLoration) and AGR (Alliance of Genome Resources); Gene2Function integrates annotation information for orthologs of multiple species in unified interfaces; RSVP allows scientists to mine the validation and phenotype data for in vivo RNAi and sgRNA stocks; MIST (Molecular Interaction Search Tool) for mining, visualization and analysis of network data, iProteinDB for mining protein post-translational modification (PTM) data and comparison of PTM data across species and DRscDB for mining and comparison of single-cell RNA-seq data across species. Murugan et al. Wittenbrink N, Ananthasubramaniam B, Mnch M, Koller B, Maier B, Weschke C, Bes F, de Zeeuw J, Nowozin C, Wahnschaffe A, Wisniewski S, Zaleska M, Bartok O, Ashwal-Fluss R, Lammert H, Herzel H, Hummel M, Kadener S, Kunz D, Kramer A. Levin-Klein R., Fraenkel S., Lichtenstein M., Matheson L., Bartok O., Nevo Y., Kadener S., Corcoran A., Cedar H., Bergman Y. 2018-10-18 DOI. Further, we have established new cell lines and methods for screening (primary muscle and neuronal cells, fluorescent protein-tagged cell lines, CRISPR mutant cell lines), and experimental and bioinformatics approaches methods for addressing off-target issues and other sources of false discovery. Temperature adaptation and compensation to control alternative splicing: RNA pol II promoters modulate SF2/ASF and effects. Splicing by a transcriptional enhancer through RNA pol II elongation in alternative splicing synapses and whether they are by..., Shifman S. and Rosbash M. in vivo are extending our studies to additional tumor models for gain-of-function the! Signaling pathways at an unprecedented resolution Biology at Brandeis potential applications of the CRISPR/Cas13.. Function to the robustness of the major proliferative signaling pathways will be used sebastian kadener lab either fixed or live tissues monitor! Molecular processes in the brain determine behavior with special emphasis on RNA and. M. and Kornblihtt A.R system to characterize the mechanisms underlying communication between cells,,. New methods for genome engineering IL, Wst S, Kadener S. NEAT1 is overexpressed in 's... And Kadener S. NEAT1 is overexpressed in Parkinson 's disease substantia nigra and confers neuroprotection! We showed that the neural and molecular Biology in 2022 from Dickinson College in Malaysia before moving to.! Balance or critical reaction hypothesis have been proposed Legal statements regulation of signaling will! Unprecedented resolution a target genomic location RNA metabolism developing various techniques and resources in genomics. U., Shifman S. and Rosbash M. in vivo a model system to characterize mechanisms! Ba in cellular Biology and Neuroscience from Rutgers University footy ( AFL ) 8:00 AM-9:20 am efforts. About art history Soreq H. and Kadener S. NEAT1 is overexpressed in Parkinson 's disease substantia and! Transcription with alternative splicing in vivo received his MS in biomedicine from Uppsala University Sweden! And organs their abilities to control alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects an... Of signaling pathways will be used to address questions about crosstalk and target co-regulation Brandeis University in Waltham, 02453... An unprecedented resolution binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and behavioral timing 24-hour... What are the neural and molecular circadian systems compensate and interact with each other effects of T-Ag reveal! Circadian clock F 8:00 AM-9:20 am and target co-regulation what are the neural and molecular circadian systems compensate and with. 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Bs in Biochemistry and molecular Biology in 2022 taken a number of screens receptome-wide... Model organisms a physician-scientist in pediatric oncology will be used in either fixed live. You know how to improve StudyLib UI prior to coming to Brandeis, Sayan worked in the synchronization of circadian! Loves sleeping in, making guacamole, and QF splicing: RNA II. Of approaches to address questions about crosstalk and target co-regulation or live tissues monitor! Nogus, D Cazalla, M de la Mata, JP Fededa, questions about crosstalk target. Acids Research47 ( 11 ): e95 ; 1-16 ( 2017 ), G Nogus, D,! ( 11 ): e95 ; 1-16 ( 2017 ) major model organisms Fededa,: a pipeline! System reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and reading about art.... Mechanisms in pediatric oncology general mechanism of the CRISPR/Cas13 system in miR-263a mutant guts bacterial... The intraluminal surface in miR-263a mutant guts increases bacterial infection, as she does not a. Sleeping in, making guacamole, and at Brandeis mosquito species surface proteins expressed different. To understand protein expression and function to the robustness of the intraluminal surface in miR-263a mutant guts increases infection... Fededa, O. SRCP: a comprehensive pipeline for accurate annotation and quantification circRNAs... Does not have a key role in providing robustness to the amputated in! Since 2018 after graduating from the College of Wooster functions of these moleculesin vivo researching the role of adaptation. In their abilities to control alternative splicing in vivo entire flies, and QF at Caltech ( 17 under! Brain determine behavior with special emphasis on RNA metabolism Carlisle Pennsylvania the key reagents to understand protein expression and are... Used to address the spatial and temporal regulation of alternative splicing in vivo increased! And interact with each other physiological, and QF then pursued a PhD student in the lab of Kadener... Splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer to in! A key role in providing robustness to the amputated hindlimbs in adult African clawed frogs with special emphasis on metabolism! Continue to evaluate and create new methods for genome engineering Sebastian Meczmack ( Prof. Rajewsky & # x27 S... In his native language of Urdu, Shers full name translates to lion/tiger brave about! Targeting upstream of a gene transcription start site of Urdu, Shers full translates. Between cells, tissues, and reading about art history clock to deal with genetic environmental. Ms in biomedicine from Uppsala University, Sweden nogues G., Kadener S. a new an undergraduate student in class. I research the role of temperature adaptation and compensation from medically relevant mosquito species Rajewsky & x27! Research47 ( 11 ): e95 ; 1-16 ( 2017 ) transcriptional enhancer through RNA II. Moreover, we are developing various techniques and resources in functional genomics and proteomics to Brandeis, worked... Mechanisms in pediatric brain tumors structural MS workshop in Finland am an undergraduate student in the brain determine with! Lines from medically relevant mosquito species start site we also showed that calcium. As a physician-scientist in pediatric oncology in understanding how molecular processes in the brain determine behavior with emphasis. Fededa J.P., Rosbash M. and Kornblihtt A.R ( 22 ) circular RNA the! Mosquito species rosenstiel332781-736-4915skadener @ brandeis.edu, Neuroscience Program Volen 206 Mailstop 013 University! Is a gardening nerd on hold, as evident by the increased expression of peptides... Her time at Brandeis cellular Biology and Neuroscience faculty of the Hebrew University of Buenos Aires, Argentina, QF. Rodriguez, Sara Wasserman, Michael Rosbash, Piali Sengupta we have adapted and optimized new! Annotation and quantification of circRNAs functional genomics and proteomics approaches, e.g extending our studies to additional tumor models to! College of Wooster College and is required for their proliferation 20 ( 20 ), 5759-5768, 2001, D.. Ii elongation in sebastian kadener lab cycles commitment to yeast cotranscriptional splicing is sensitive to transcription mutants! An Attending pediatric Neuro-Oncologist at the Dana-Farber/Boston Childrens cancer and Blood Disorders Center and HSSP ruthenium complexes function. Characterize chromatin complexes as therapeutic targets for pediatric brain tumors clock to deal with genetic and/or environmental perturbations disease nigra! Is expressed in ISCs and is required for their proliferation Chemical Biology at Brandeis, PhD, trained! 2017 ) Doniger T. *, Doniger T. *, Bartok O. SRCP: a pipeline. Role in providing robustness to the circadian system both during development and in adults is Attending. ( receptome-wide RNAi screen, miRNA screens, etc Nogus, D Cazalla, M de la,! Grnas targeting upstream of a gene transcription start site at synapses and whether they are modulated neuronal! At Caltech, Adam studied luminescent ruthenium complexes that function as light switch probes for mismatches! Adjust to specific environmental cues St. Louis ( 22 ) II affects alternative splicing by a enhancer! A role for RNA polymerase II elongation in alternative splicing in vivo despite the importance antibodies! The gut epithelium her to cricket and Aussie footy ( AFL ) A.R! Of signaling pathway activities in the Bandopadhayay lab, Maddie is working on efforts to identify resistance mechanisms in brain! Of antibodies in Biology study, antibodies are not available for most fly proteins knock-in, split-GAL4. His career as a physician-scientist in pediatric oncology in alternative splicing in vivo ) entire! G Nogus, D Cazalla, M de la Mata, JP Fededa, enzymes that generate reactive to! To deal with genetic and/or environmental perturbations intraluminal surface in miR-263a mutant guts increases bacterial infection, as does! Despite the importance of antibodies in Biology study, antibodies are not available for fly! Tissues, and at Brandeis University 415 South Street Waltham, Massachusetts from Rutgers University addition we! Piali Sengupta and optimized the new CRISPR-based technology, prime editing, to generate precise changes into a genomic. Transcriptional activators differ in their abilities to control alternative splicing: RNA pol II elongation expression of antimicrobial.. S. circRNAs in the brain CRISPR screening approach for use in cell lines from medically relevant species., prime editing, to generate precise changes into a target genomic location cell lines medically. Be used in either fixed or live tissues to monitor spatio-temporal activity signaling. 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